Molecular characterization and role in T cell activation of staphylococcal enterotoxin A binding to the HLA-DR alpha-chain

Superantigens bind to MHC class II-positive cells and stimulate T lymphocytes expressing specific V beta regions of the TCR. Two distinct regions of staphylococcal enterotoxin A superantigen (SEA) have been shown to affect the binding to MHC class II molecules. Results presented here demonstrate for the first time that the SEA-DR interaction can be affected by mutations on the class II alpha-chain. Furthermore, we have precisely mapped the interaction of the SEA N-terminal domain with the alpha1 domain of HLA-DR. Scatchard analysis using DAP cells transfected with mutant class II molecules showed a role for residue DR alpha K39 in the binding of SEA. Also, complementation experiments using mutant SEA molecules revealed an interaction between SEA residue F47 and position alphaQ18 on an outer loop of HLA-DR. These interactions between SEAF47 and the DR alpha-chain are critical, as they allow the recognition by an otherwise nonreactive V beta1+ T cell hybridoma and induction of tyrosine phosphorylation through the TCR.     

A 3.3 V, 10 Bits, Clock-Feedthrough Compensated Switched-Current Second Order Sigma-Delta Modulator

This article presents a low-pass sigma-delta modulator for Analogue-to-Digital conversion. The circuit uses a switched-current technique which presents a well known drawback called clock feedthrough. This phenomenon induces an error on the output signal value. In order to cancel the clock feedthrough effect, we use a new method based on a current feedback loop. The circuit is designed in 0.8 μm AMS “Austria Mikro Systems” single poly CMOS process. Measurements of the modulator are performed under A/D converters characterisation system, and show 55 dB dynamic range at 2.048 MHz sampling rate with 8 kHz input frequency bandwidth. These characteristics are suitable for audio applications.     

Serial renal transplant surgery: technical reflections concerning third transplants

Iodine distribution was studied in isolated porcine eyes after iontophoresis using 131I-labelled solutions and chemical methods. After a 15-min treatment with a therapeutically used iodine brine, the iodine content in the ocular tissues showed the following rank order: cornea > retina > vitreous body > anterior chamber fluid > lens. Replacement of the brine by 0.15 M NaI increased the uptake values in a different way, but the same rank order was maintained. The increase was highest in the cornea (5.6-fold) and only about 2-fold in the lens, nevertheless approaching now the level of 10(-4) M, a concentration which was regarded as being optimal for the antioxidant effect of I-. Among the other eye components, the relatively high iodine enrichment of the retina was remarkable. The data are discussed with respect to a possible protective, antioxidative and OH-scavenging efficacy of I- and to previous results in the literature concerning iodine uptake in ocular tissues.     

Measurement of the vascular mass of the human radial artery by ultrasonics

OBJECTIVE: To determine the reproducibility of radial artery mass (RAM), in hypertensive patients. DESIGN AND METHODS: In 49 patients, RAM was measured using a high resolution echotracking device (Nius-02) which allows noninvasive measurement of diameter and wall thickness of the radial artery. RAM was validated in vitro by comparing weight of arterial segments to ultrasonographic measurement and determined as RAM = r (pi Re2-pi Ri2) where r is the arterial wall density (1.06 g/cm3), and Re and Ri are values of internal and external radii, respectively. Repeatability coefficient (RC2 = SDi2/n) was 1.3 mg. RESULTS: Blood pressure was (mean +/- SD) 146 +/- 19/85 +/- 15 mmHg, radial arterial diameter was 2,449 +/- 376 microns, radial wall thickness was 302 +/- 68 microns, RAM was 28 +/- 9 mg (range 13-43 mg). CONCLUSION: These results indicate that radial artery mass can be measured using a high resolution echotracking device.     

Converting-enzyme inhibitor versus calcium antagonist in cyclosporine-treated renal transplants

The influence of antihypertensive treatment on the long-term evolution of arterial pressure and renal function was studied in a prospective controlled trial conducted in renal transplant recipients treated by cyclosporine. Within six months after transplantation, patients were randomly allocated to treatment by the angiotensin-converting enzyme inhibitor, lisinopril (ACEI, alone or associated with frusemide; N = 14), or the calcium antagonist, nifedipine (CA, alone or associated with atenolol; N = 11). Glomerular filtration rate (TcDTPA clearance) and effective renal plasma flow (hippuran clearance) as well as 24-hour urinary excretion of electrolytes and albumin were estimated at about 1 and 2.5 years of follow-up. Before initiation of antihypertensive therapy, the two groups were similar with regards to mean arterial pressure (119 +/- 2 vs. 120 +/- 4 mm Hg), effective renal plasma flow (285 +/- 26 vs. 248 +/- 33 ml/min/1.73 m2) and glomerular filtration rate (59 +/- 4 vs. 61 +/- 8 ml/min/1.73 m2 in the ACEI and CA groups, respectively). Both ACEI and CA treatments were associated with no change in renal function, a similar change in mean arterial pressure (ACEI -18 +/- 3; CA -13 +/- 5 mm Hg) and identical trough blood levels of cyclosporine. Urinary albumin excretion did not change significantly in any groups. Of interest, only in the ACEI group did filtration fraction significantly decrease (from 0.22 +/- 0.01% to 0.19 +/- 0.01% at final studies). These results indicate that in cyclosporine-treated transplant recipients, a satisfactory control of hypertension is obtained by chronic ACEI, which is as effective on arterial pressure as a combination of CA and atenolol.(ABSTRACT TRUNCATED AT 250 WORDS)     

MODE II STABLE CRACK GROWTH

Abstract Mode II stable crack extension has been examined for an aircraft grade aluminium alloy D16AT. Both theoretical and experimental results are presented. The experimental observations include load displacement diagrams, plastic wake, crack front tunnelling and scanning electron micrographs of the fracture surfaces. The crack shows a tendency for in-plane extension, and the fracture surface is very flat, smooth and free of any dimples. The crack front advances with neghgible tunnelling at all stages of extension. The span of mode II stable crack growth (SCG) is longer than in the case of mode I SCG reported earlier for the same material and there is also more extensive plastic deformation. In the presence of a slight mode I load, the crack grows out-of-plane and the fractured surface facets resemble that of a mode I or mixed-mode dimpled fracture. The theoretical study is based on a finite element analysis using small deformation theory and incremental plasticity. Some of the experimental results have been theoretically predicted using the COA criterion as the governing criterion. The theoretical results include load-displacement diagrams, crack edge displacement curves, plastic zones and the J resistance curves. There is good agreement between the load-displacement diagrams. The initiation and maximum loads differ by less than 15%. The J resistance curve has a constant slope over the whole span of stable crack growth.     

A novel classification approach based on Extreme Learning Machine and Wavelet Neural Networks

Multimedia Tools and Applications - In this paper, we present a novel classification approach based on Extreme Learning Machine (ELM) and Wavelet Neural Networks. We introduce two novel...     

A passivity‐based simple adaptive synergetic control for a class of nonlinear systems

For a class of nonlinear systems with the representation {A(x), B(x), C} and where the system parameters and dynamics are unknown, a simple adaptive synergetic controller ensuring the asymptotic convergence of the system to a desired manifold is proposed based on the technique of simple adaptive control (SAC). It is well known that the design of the synergetic control (SC) law requires a thorough knowledge of the system parameters and dynamics. Such problem obstructs the synthesis of the SC law and the designer is prompted to pass through the estimation methods, which, in turn, poses a problem of increasing the computation time of the control algorithm. To cope with this problem, a solution is proposed by modifying the original SC law to develop an SAC‐like adaptive SC law without the need of prior knowledge of the system. The stability of the proposed adaptive controller is formally proven via the Lyapunov approach. Experimental application to a quadrotor system is given to validate the theoretical results.